‘The accumulation of highly differentiated Mtb-specific CD4 T cells is associated with lung tissue destruction during pulmonary tuberculosis.’
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1
Central Tuberculosis Research Institute, Russia
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2
University of Tennessee, United States
Immunological methods are widely used for the diagnostics of M. tuberculosis (Mtb) infection. In this study we show that these methods can also be used as a means to evaluate and monitor the degree of lung tissue destruction caused by Mtb. Lung destruction is a characteristic trait of pulmonary tuberculosis (TB). It underlies disease severity, causes dissemination of the pathogen and needs to be carefully evaluated. Our earlier studies in mice have demonstrated that Mtb infection induces the differentiation of effector CD4 T cells into highly differentiated CD4+CD27- lymphocytes and that this differentiation takes place in the lungs. We hypothesized therefore that the accumulation of CD4+CD27- cells should mirror tuberculosis activity. In this study, we evaluated the percent of CD27- cells within the population of Mtb-reactive CD4 T cells (“Mtb+CD27-“ cells) in the blood of TB patients.
Compared to healthy donors, TB patients had significantly higher percent of Mtb+CD27- cells (p<0.0001). Within the group of TB patients, the percent of Mtb+CD27- cells varied. Among different characteristics of TB disease (i.e., pulmonary destruction, disease duration, et al.), high degree of pulmonary destruction was the main factor associated with high percent of Mtb+CD27- cells (p<0.0001). ROC-curve analysis revealed that evaluation of Mtb+CD27- cells allows assessing pulmonary destruction (sensitivity, 89%; specificity, 74%). During the treatment, a decrease in the percent of Mtb+CD27- cells was indicative on the repair of lung destruction (p<0.01).
The determination of the percent of Mtb+CD27- cells allows assessing pulmonary destruction and its repair following TB therapy.
Support: NIH (#A1078899)
Keywords:
T-cell immunity,
CD4 T cells,
differentiation,
Tuberculosis,
Diagnosis, Differential,
Flow Cytometry
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Nikitina
I,
Amansahedov
R,
Panteleev
A,
Ganusov
V,
Vasilyeva
I and
Lyadova
I
(2013). ‘The accumulation of highly differentiated Mtb-specific CD4 T cells is associated with lung tissue destruction during pulmonary tuberculosis.’.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00222
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Received:
14 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mrs. Irina Nikitina, Central Tuberculosis Research Institute, Moscow, Russia, redwings2009@yandex.ru