Cytokine polymorphisms, their influence and levels in Brazilian patients with pulmonary tuberculosis during anti-tuberculosis treatment.
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1
São Paulo State University - UNESP, Brazil
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2
Lauro de Souza Lima Institute, Brazil
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3
The Methodist Hospital Research Institute, United States
Cytokines play an essential role during active tuberculosis disease and cytokine genes have been described in association with altered cytokine levels. Therefore, the aim of this study was to verify if IFNG, IL12B, TNF, IL17A, IL10 and TGFB1 gene polymorphisms (SNP) influence the immune response of Brazilian patients with pulmonary tuberculosis (PTB) at different time points of anti-tuberculosis treatment: T1: beginning; T2: three months; and T3: six months. Our results showed that the IFNG+874 T allele and the IFNG+2109 A allele were associated with higher IFN-gamma levels. The IL12B+1188 C allele was associated with higher IL-12 levels. A TNF-308 A allele was associated with higher TNF-alpha plasma levels in controls and mRNA levels in PTB patients at T1. The IL17A A allele at rs7747909 was associated with higher IL-17 levels. The IL10-819 T allele was associated with higher IL-10 levels. A TGFB1+29 CC genotype was associated with higher TGF-beta plasma levels in PTB patients at T2. The present study suggests that IFNG+874T/A, IFNG+2109A/G, IL12+1188A/C, IL10-819C/T and TGFB1+21C/T are associated with different cytokine levels in PTB patients and may play a role in the initiation and maintenance of acquired cellular immunity to TB and in the outcome of the active disease and anti-tuberculosis treatment. Cytokine profile determined by the respective functional SNPs could demonstrate the actual pattern of the cytokine response against the mycobacteria and may provide a better understanding of active TB disease progression and response to the anti-TB treatment and may serve as genetic risk markers of TB susceptibility.
Acknowledgements
This study was funded by Grants from “Fundação de Amparo à Pesquisa do Estado de São Paulo” – FAPESP, Brazil.
Keywords:
pulmonary tuberculosis,
Single nucleotide polymorphisms,
cytokine,
anti-tuberculosis treatment,
ELISA
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Peresi
E,
Oliveira
LR,
Da Silva
WL,
Da Costa
EA,
Araujo Junior
JP,
Ayres
JA,
Fortes
MP,
Graviss
EA,
Pereira
A and
Calvi
SA
(2013). Cytokine polymorphisms, their influence and levels in Brazilian patients with pulmonary tuberculosis during anti-tuberculosis treatment..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00202
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Received:
13 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Eliana Peresi, São Paulo State University - UNESP, Botucatu, Brazil, elianaperesi@yahoo.com.br