Molecular mechanisms for memory B-cell development and function
-
1
Tokyo University of Science, Research Institute for Biomedical Sciences, Japan
-
2
Duke University, Department of Immunology, United States
Upon immunization with T-cell dependent antigens, antigen-bound B cells proliferate extensively to form germinal centers (GC) in the peripheral lymphoid organs and then differentiate into either long-lived plasma cells (LLPCs) or memory B (Bmem) cells, both of which constitute a B-cell part of the immunological memory. However, molecular mechanisms for the development of Bmem cells remain poorly understood, partly due to the lack of an in vitro model system. Recently, we have established a culture system in which mouse naïve B cells can be extensively propagated on feeder cells expressing CD40L and BAFF to generate GC-phenotype B (iGB) cells undergoing immunoglobulin class switching. The iGB cells after primary culture with IL-4 develop into Bmem cells in vivo that elicit rapid immune responses, whereas after the secondary culture with IL-21, they develop in vivo into LLPCs that produce antibodies in the bone marrow, but not Bmem cells. The tertiary culture without cytokines partially restores the Bmem development and abolishes the LLPC development. Thus, this novel system has enabled in vitro differentiation from naïve B cells into Bmem or LLPC precursors that maturate in vivo, and therefore will be useful to study the mechanisms for the development and function of Bmem and LLPCs. In this workshop, I will discuss newly discovered molecular requirements for the Bmem cell development and function.
Keywords:
memory B cells,
Germinal Center,
Plasma Cells,
Immune responses,
Immunological memory
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Kitamura
D,
Haniuda
K,
Fukao
S,
Horiuchi
S,
Takatsuka
S,
Moutai
T and
Nojima
T
(2013). Molecular mechanisms for memory B-cell development and function.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00181
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
11 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Daisuke Kitamura, Tokyo University of Science, Research Institute for Biomedical Sciences, Noda, Chiba, 278-0022, Japan, kitamura@rs.noda.tus.ac.jp