B cell antigen presentation induces Tfh-differentiation from Th2 cells in vitro
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1
Research Institute for Biomedical Science Tokyo University of Science, Japan
During T-dependent immune responses, after antigen presentation from B cells to CD4 T cells, B cells proliferate extensively to form germinal center (GC), and CD4 T cells differentiate into distinct effector helper T cell subsets, such as Th1, Th2, and follicular helper T (Tfh) cells. To clarify the mechanisms of the differentiation of B cells and CD4 T cells, we analyzed B cells and T cells co-cultured in the culture system we have recently established, in which B cells are cultured on feeder cells expressing CD40 ligand and BAFF. By using this culture system, we show that, when cultured with naïve B cells in the presence of antigens under the Th2 conditions (i.e. with IL-2 and IL-4), in-vitro-derived Th2 cells differentiate into cells with Tfh-like phenotype (CXCR5+ PD-1+ ICOS+ and IL-21 producing). Surprisingly, these Th2-derived Tfh-like cells retained the function of producing IL-4 and expressed GATA3 mRNA. On the other hand, B cells cultured with Th2 cells, in the presence of antigens, differentiate into cells with pre-memory like phenotype (B220+ CD38+ FAS+ GL7+) and plasma cells (Blimp1+ CD138+). Thus, we demonstrate efficient in vitro differentiation of Th2 cells into Tfh-like cells that also retain the nature of Th2 cells, which are reminiscent to a recently proposed Tfh subset. We also show that antigen-presenting B cells are necessary for the differentiation of Tfh-like cells.
Keywords:
Tfh cell,
Helper T cells,
B cells,
Immunological memory,
Germinal Center
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Horiuchi
S,
Takebuchi
H,
Konishi
S,
Haniuda
K,
Moutai
T,
Takatsuka
S and
Kitamura
D
(2013). B cell antigen presentation induces Tfh-differentiation from Th2 cells in vitro.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00122
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Received:
11 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Daisuke Kitamura, Research Institute for Biomedical Science Tokyo University of Science, Chiba, Japan, kitamura@rs.noda.tus.ac.jp