Downregulation of IL-17-producing T cells is associated with regulatory T cell expansion and disease progression in chronic lymphocytic leukemia
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1
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Iran
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2
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Iran
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3
Clinic of Hematology and Oncology, Firozgar Hospital, Faculty of Medicine, Tehran University of Medical Sciences, Iran
Little is known about the immunobiology of interleukin-17 (IL17) producing T cells and regulatory T cells (Treg) in chronic lymphocytic leukaemia (CLL). In this study, the frequencies of Th17, Tc17 and CD39+Treg cells were enumerated in peripheral T cells isolated from 40 CLL patients and 15 normal subjects by flow cytometry. Our results showed a lower frequency of Th17 and Tc17 cells in progressive (0.99±0.12% of total CD3+CD4+ cells, 0.44±0.09% of total CD8+ cells) compared to indolent patients (1.57±0.24%, p=0.042, 0.82±0.2%, p=0.09) and normal subjects (1.78±0.2%, p=0.003, 0.71±0.09%, p=0.04). Decrease in IL17 producing T cells was associated with CD39+Treg cells expansion. Variation of IL17 producing cells and Treg cells in indolent and progressive patients was neither associated to the expression levels of Th1 and Th2 specific transcription factors T-bet and GATA-3 nor to the frequencies of IFNγ and IL4 producing CD4+ T cells in a selected number of samples. Additionally, suppressive potential of CD4+ Treg was similar in CLL patients and normal subjects. Our data indicates that progression of CLL is associated with downregulation of IL17 producing T cells and expansion of Treg cells, implying contribution of these subsets of T cells in progression of CLL.
Acknowledgements
We would like to thank, Mehdi Yousefi, Tahereh Shahrestani and Bita Ansaripour for their excellent technical support. This study was supported in part by a grant from Tehran University of Medical Sciences (grant number: 9879).
Keywords:
Th17,
Tc17,
Regulatory T Cell,
chronic lymphocytic leukaemia,
Interleukin 17
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Jadidi-Niaragh
F,
Ghalamfarsa
G,
Memarian
A,
Asgarian-Omran
H,
Razavi
S,
Sarrafnejad
A and
Shokri
F
(2013). Downregulation of IL-17-producing T cells is associated with regulatory T cell expansion and disease progression in chronic lymphocytic leukemia.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00084
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Received:
09 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mr. Farhad Jadidi-Niaragh, Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, farhad_jadidi1986@yahoo.com
Prof. Fazel Shokri, Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, fshokri@tums.ac.ir