Methylation patterns near and inside of the TSLP promoter in an African descendent population of Colombia
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1
Universidad de Cartagena, Instituto de Investigaciones Inmunológicas, Colombia
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2
Universidad de Antioquia, Instituto de Biología, Colombia
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3
Genes LTDA., Colombia
Rationale: Thymic stromal lymphopoietin - TSLP is a gene that affects many cell lineages and can directly or indirectly leads to various outcomes, the expression of it is detected in the airways of asthmatic patients, and correlates with disease severity. For this reason we decided to analyze the methylation patterns of the TSLP in Afro-Colombian population.
Methods: We analyze the methylation patterns close and inside of the TSLP promoter in 94 African-descent individuals of Colombian Caribbean coast. From TSLP isoform 1 precursor sequence reported by NCBI, we selected the sequence from chromosomal location chr5: 110405778 to 110413722, that containing 5’UTR and promoter regions from TSLP. Using Methprimer the parameters for prediction of CpG Islands were CpG islands >100bp, GC>50% and Obs/Exp >0.60.
Results: Methprimer analysis predicted two CpG Islands, where primers for methylation specific PCR (MSP) where designed, one in the 5’UTR region (Island 1 with 190bp) and the other one in his promoter (Island 2 with 231bp). We found, that Island 1 showed a variable methylation pattern with unmethylated, methylated and hipermethylated individuals (24.5%, 44.7% and 30.9%, respectively. While, the Island 2 was almost always hipermethylated (92.6%, n=87).
Conclusions: Although this study was performed with peripheral blood samples, the Island 1 in the 5’UTR is more informative than Island 2, and could be regulating gene expression of TSLP and may be associated with the levels of TSLP protein in serum.
Acknowledgements
The authors acknowledge to the Universidad de Cartagena for their financial support in developing this study, and Colciencias for its young research program which favored with a scholarship-internship for participating master's students. Also to all those who voluntarily and unconditionally helped in the development of this study.
References
Fang L, Martínez B, Marrugo J. Distribución de tres polimorfismos del gen TSLP en población Afrodescendiente de San Basilio de Palenque, Colombia. Biomedica. 2013; 33(2): [on line].
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Gao PS, Rafaels NM, Mu D, Hand T, Murray T, Boguniewicz M, et al. Genetic variants in thymic stromal lymphopoietin are associated with atopic dermatitis and eczema herpeticum. J Allergy Clin Immunol. 2010;125(6):1403-7 e4.
Sherrill JD, Gao PS, Stucke EM, Blanchard C, Collins MH, Putnam PE, et al. Variants of thymic stromal lymphopoietin and its receptor associate with eosinophilic esophagitis. J Allergy Clin Immunol. 2010;126(1):160-5 e3.
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Keywords:
thymic stromal lymphopoietin,
DNA Methylation,
Promotor Region,
5’UTR.,
Epigenetic Process
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune receptors and signaling
Citation:
Fang
L,
Manrique Rincón
AJ,
Builes Gómez
JJ,
Hernandez
L,
Aguirre
DP,
Muñoz
C,
Martínez
B and
Marrugo
J
(2013). Methylation patterns near and inside of the TSLP promoter in an African descendent population of Colombia.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00010
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Received:
09 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mrs. Beatriz Martínez, Universidad de Cartagena, Instituto de Investigaciones Inmunológicas, Cartagena, Bolivar, Colombia, bmartineza@unicartagena.edu.co