Impairment of food intake and lipid metabolism by DEHP in zebrafish
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1
Polytechnic University of Marche, Department of Marine Sciences, Italy
Toxicological and epidemiological studies have suggested the involvement of different environmental chemicals in increasing metabolic disorders. Evidence points to endocrine disruptors that interfere with cellular biology of the adipose tissue and central hypothalamic-pituitary-gonadal/adrenal axis derailing the pathways involved in homeostatic and metabolic control.
DEHP is an ester of phthalic acid utilized for the production of several plastic products. For this plasticizer, an adverse effect on lipid metabolism has been described in rats, in terms of increase of fatty acid oxidation and lipid mobilization atrophy. Nevertheless, very few information are still available regarding DEHP potential impairment of food intake. Thus, in this study, DEHP capability to induce metabolic disorders was verified by relating its effect on lipid metabolism and food intake in zebrafish.
A number of metabolic regulators critical for lipid metabolism such as PPARa, SREBP and CB1 were analyzed in zebrafish liver by Real time PCR. Food intake was measured and the gene expression of key molecules involved in the central control of appetite (NPY, orexin, CB1, SREBP and leptin) was tested.
The results indicated an estrogenic and adverse effect of DEHP on feeding behaviour which is maybe due to altered levels of signals controlling hepatic de novo fatty acid synthesis. In particular, the lowest dose tested resulted to be the most dangerous thus stressing the importance to focus on the regulation on pollutants emission in the environment.
Keywords:
comparative endocrinology
Conference:
25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010.
Presentation Type:
Conference Presentation
Topic:
Comparative endocrinology
Citation:
Migliarini
B,
Piccinetti
CC,
Martella
A,
Maradonna
F,
Tosti
L and
Carnevali
O
(2010). Impairment of food intake and lipid metabolism by DEHP in zebrafish.
Front. Endocrinol.
Conference Abstract:
25th Conference of the European Comparative Endocrinologists.
doi: 10.3389/conf.fendo.2010.01.00009
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Received:
26 Aug 2010;
Published Online:
29 Aug 2010.
*
Correspondence:
Dr. Beatrice Migliarini, Polytechnic University of Marche, Department of Marine Sciences, Ancona, Italy, b.migliarini@univpm.it