The diversity of chromosome structure of males in Cobitis (Teleostei, Cobitidae) populations
Alicja
Boron1*,
Anna
Grabowska1,
Aneta
Spoz1,
Dorota
Juchno1,
Anna
Leska1,
Olga
Jablonska1,
Anna
Przybył1,
Aleksandra
Szabelska1,
Karolina
Kowalewska1,
Anna
Pecio2,
Beata
Cejko3 and
Radosław
Kowalski3
-
1
University of Warmia and Mazury in Olsztyn, Department of Zoology, Poland
-
2
Institute of Zoology, Department of Comparative Anatomy, Poland
-
3
Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland
The populations of Cobitis distributed in Poland are usually composed of the spined loach C. taenia Linnaeus, 1758 (2n=48) or the danubian loach C. elongatoides Bacescu & Maier, 1969 (2n=50), triploid hybrid females (3n=73, 3n=74, 3n=75) and tetraploid (4n=98, 4n=99) hybrids of both sexes. The results of our experimental studies reveal that c. 60% of eggs of allotriploid females dominating in these populations may be fertilized by C. taenia males whereas the tetraploid Cobitis males are sterile. The aim of this study was to show the chromosome structure of diploid and polyploid Cobitis males distributed in diploid and diploid-polyploid populations in Polish water bodies.
Chromosomes of C. taenia and C. elongatoides males (each of 10 individuals) and of tetraploid Cobitis males (12 individuals) were examined using double-colour fluorescence in situ hybridisation (FISH) with 28S and 5S rDNA probes. In the karyotype of C. taenia and C. elongatoides respectively the modal number of nine and six, and seven and two, respectively of 28S rDNA and 5S rDNA sites were detected. C. taenia and C. elongatoides characterized respectively by four and two chromosomes containing hybridization sites of both rDNA probes (co-localizations). The chromosome sets of 4n males were composed of the C. taenia, C. elongatoides and C. tanaitica genomes. The pattern of rDNA sites distribution indicated that apart others the karyotypes of 4n males were composed of one or two haploid chromosome set of C. elongatoides.
The presented data give an important insight into the structure of chromosomes of two Cobitis species and into the genome structure of allotetraploid Cobitis males. The obtained result is discussed with previously published studies.
Acknowledgements
The project was financed by the National Science Centre and was obtained on the basis of Decision DEC-2011/03/B/NZ8/02982, and in part by Project No. 12.610.006-300 financed by UWM in Olsztyn.
Keywords:
Polyploidy,
chromosome structure,
FISH with rDNA probes,
Cobitis,
genome identification
Conference:
XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015.
Presentation Type:
Oral Presentation
Topic:
Phylogeny, Systematics and Genetics
Citation:
Boron
A,
Grabowska
A,
Spoz
A,
Juchno
D,
Leska
A,
Jablonska
O,
Przybył
A,
Szabelska
A,
Kowalewska
K,
Pecio
A,
Cejko
B and
Kowalski
R
(2015). The diversity of chromosome structure of males in Cobitis (Teleostei, Cobitidae) populations.
Front. Mar. Sci.
Conference Abstract:
XV European Congress of Ichthyology.
doi: 10.3389/conf.fmars.2015.03.00023
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Received:
26 Oct 2015;
Published Online:
04 Nov 2015.
*
Correspondence:
Prof. Alicja Boron, University of Warmia and Mazury in Olsztyn, Department of Zoology, Olsztyn, 10-718, Poland, alibo@uwm.edu.pl