Introduction: Plasma polymerization has been used in different works as a good surface treatment for cell interaction it has work with several kinds of cells and our laboratory has work specially with polymer of pyrrole synthesized by plasma (PPy)[1]-[3]. The chemical diversity of the surface gives opportunity for protein interaction and therefore for cell anchor. Also, we have synthesized plasma pyrrole nanoparticles (PPNP) that present the same chemical variety of the films in the surface[4]. In this work we present the study of the interaction of PPNP with two cell lines, the particles allow cell viability on aggregates that can go from a couple of cells to microtissues, giving sense to the term nanoparticle-scaffold.
Material and Methods: PPy nanoparticles were synthesized in a cylindrical plasma reactor connected with a radio frequency source at 13.56 MHz, as reported elsewhere[4]. The cell lines RIN-M and NG 108-15 ware used. Each one of the cell lines cultures was divided in two groups, one group without PPy nanoparticles (control) and the other group with PPy nanoparticles. The two groups were cultivated in suspension with a humidity controlled incubator at 37oC and 5% CO2.. PPNP were characterized by Fourier transform infrared spectroscopy (FTIR-ATR) and scanning electron microscopy (SEM), Cell cultures were characterized with optical microscopy and cofocal microscopy.
Results: Both cell lines began to adhere to the PPy nanoparticles a few minutes after the culture was started and continued adhered along the time from a couple of cells to microtissues. The PPy nanoparticles promoted cell survival in both cell lines compared to the control group. Nanoparticles aggregates also promoted larger cell agglomerations and they also keep them viable.
Discussions:
Others biomaterials used in suspension culture reported times of cellular adhesion of a few hours[5] to a week[6], PPNP were kept adhered for at least up to three weeks, kipping viable cells.K. Sojin el al[7]. showed that polypirrole nanoparticles synthesized by water-soluble polymer/metal cation system have cytotoxic effects on cell lines, in contrast with their report we found that the PPy nanoparticles helped to cell survival in the cell lines used.
Conclusion: PPy nanoparticles synthesized by plasma have adhesion features and helped to cell survival therefore it may have potential applications in biomedical engineering.
CONACYT
References:
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