Bioactive injectable hydrogel conjugated with a synthetic peptide for bone regeneration
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1
School of Dentistry, Seoul National University, Department of Dental Regenerative Biotechnology, Korea
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2
Ajou University, Department of Molecular Science and Technology, Korea
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3
School of Medicine, Ewha Womans University, Department of Molecular Medicine, Korea
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4
Nano Intelligent Biomedical Engineering Corporation (NIBEC), Central Research Institute, Korea
Hard tissue engineering is a developing field pursued at satisfying the needs for the restoration of damaged bone or missed teeth. To achieve the aim, an ideal three-dimensional scaffold should need to provide a mimetic environment according to the original extracellular matrix of hard tissues. Because it is not sufficient to regenerate the targeted tissues with scaffold alone, however, design and fabrication of proper scaffold for bone regenerative engineering have been considered that used with bioactive molecules such as peptides. Herein, the bioactive peptide (Pep) for biomineralization was identified from Osteopontin (OPN) described as a major non-collagenous protein in bone, which influences bone homeostasis both by promoting differentiation of osteoblasts and by enhancing osteoblast activity. In this study, we investigated in vivo long-term localization and effective hard tissue regeneration of human mesenchymal stem cells using enzymatically triggered conjugating system. In brief, the bioactive hydrogels were prepared by conjugating with Pep including the tyrosine residue via an enzyme-mediated reaction, and the cells were incorporated simultaneously into the gels. Taken together, the injectable osteoinductive scaffold might be a potent bone regenerative material due to easier structure formation and more adjustable release of bioactive molecules than conventional regenerative biomaterials.
This work was supported in part by the the Bio & Medical Technology Development Program (#NRF-2014M3A9E3064431, #NRF-2014M3A9E3064433 and #NRF-2013R1A1A3007591) and Basic Science Research Program (#NRF-2015M3A9E2028577 and #NRF-2015R1A2A14027221) through the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning.
References:
[1] Choi YJ, Lee JY, Chung CP and Park YJ, “Enhanced osteogenesis by collagen-binding peptide from bone sialoprotein in vitro and in vivo”, J Biomed Mater Res A., Vol. 101A, Issue 2, (2013), pp 547-554.
[2] Park KM, Lee Y, Son JY, Bae JW and Park KD, “In situ SVVYGLR peptide conjugation into injectable gelatin-poly(ethylene glycol)-tyramine hydrogel via enzyme-mediated reaction for enhancement of endothelial cell activity and neo-vascularization”, Bioconjug Chem., Volume 23, Issue 10, (2012), pp 2042-2050
Keywords:
Hydrogel,
Tissue Regeneration,
in vivo tissue engineering,
Bioactive molecule
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Regenerative medicine: biomaterials for control of tissue induction
Citation:
Suh
J,
Jo
B,
Lee
Y,
Park
Y,
Lee
J,
Park
K,
Chung
C and
Park
Y
(2016). Bioactive injectable hydrogel conjugated with a synthetic peptide for bone regeneration.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.00421
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.